Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate.
Identifieur interne : 000038 ( Main/Exploration ); précédent : 000037; suivant : 000039Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate.
Auteurs : Gabriel Bidaux [France] ; Anne-Sophie Borowiec [France] ; Natalia Prevarskaya [France] ; Dmitri Gordienko [France]Source :
- Channels (Austin, Tex.) ; 2016.
Abstract
Recently, we reported the cloning and characterization of short isoform of the icilin-activated cold receptor TRPM8 channel in keratinocytes, dubbed eTRPM8. We demonstrated that eTRPM8 via fine tuning of the endoplasmic reticulum (ER) - mitochondria Ca(2+) shuttling regulates mitochondrial ATP and superoxide (O2(•-)) production and, thereby, mediates control of epidermal homeostasis by mild cold. Here, we provide additional information explaining why eTRPM8 suppression and TRPM8 stimulation both inhibit keratinocyte growth. We also demonstrate that stimulation of eTRPM8 with icilin may give rise to sustained oscillatory responses. Furthermore, we show that ATP-induced cytosolic and mitochondrial Ca(2+) responses are attenuated by eTRPM8 suppression. This suggests positive interplay between eTRPM8 and purinergic signaling pathways, what may serve to facilitate the ER-mitochondria Ca(2+) shuttling. Finally, we demonstrate that cold (25°C) induces eTRPM8-dependent superoxide-mediated necrosis of keratinocytes. Altogether, these results are in line with our model of eTRPM8-mediated cold-dependent balance between keratinocyte proliferation and differentiation.
DOI: 10.1080/19336950.2016.1168551
PubMed: 27014839
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000005
- to stream PubMed, to step Curation: 000005
- to stream PubMed, to step Checkpoint: 000025
- to stream Ncbi, to step Merge: 000F04
- to stream Ncbi, to step Curation: 000F04
- to stream Ncbi, to step Checkpoint: 000F04
- to stream Main, to step Merge: 000038
- to stream Main, to step Curation: 000038
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate.</title><author><name sortKey="Bidaux, Gabriel" sort="Bidaux, Gabriel" uniqKey="Bidaux G" first="Gabriel" last="Bidaux">Gabriel Bidaux</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Borowiec, Anne Sophie" sort="Borowiec, Anne Sophie" uniqKey="Borowiec A" first="Anne-Sophie" last="Borowiec">Anne-Sophie Borowiec</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Prevarskaya, Natalia" sort="Prevarskaya, Natalia" uniqKey="Prevarskaya N" first="Natalia" last="Prevarskaya">Natalia Prevarskaya</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Gordienko, Dmitri" sort="Gordienko, Dmitri" uniqKey="Gordienko D" first="Dmitri" last="Gordienko">Dmitri Gordienko</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2016">2016</date><idno type="doi">10.1080/19336950.2016.1168551</idno><idno type="RBID">pubmed:27014839</idno><idno type="pmid">27014839</idno><idno type="wicri:Area/PubMed/Corpus">000005</idno><idno type="wicri:Area/PubMed/Curation">000005</idno><idno type="wicri:Area/PubMed/Checkpoint">000025</idno><idno type="wicri:Area/Ncbi/Merge">000F04</idno><idno type="wicri:Area/Ncbi/Curation">000F04</idno><idno type="wicri:Area/Ncbi/Checkpoint">000F04</idno><idno type="wicri:Area/Main/Merge">000038</idno><idno type="wicri:Area/Main/Curation">000038</idno><idno type="wicri:Area/Main/Exploration">000038</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate.</title><author><name sortKey="Bidaux, Gabriel" sort="Bidaux, Gabriel" uniqKey="Bidaux G" first="Gabriel" last="Bidaux">Gabriel Bidaux</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Borowiec, Anne Sophie" sort="Borowiec, Anne Sophie" uniqKey="Borowiec A" first="Anne-Sophie" last="Borowiec">Anne-Sophie Borowiec</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Prevarskaya, Natalia" sort="Prevarskaya, Natalia" uniqKey="Prevarskaya N" first="Natalia" last="Prevarskaya">Natalia Prevarskaya</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author><author><name sortKey="Gordienko, Dmitri" sort="Gordienko, Dmitri" uniqKey="Gordienko D" first="Dmitri" last="Gordienko">Dmitri Gordienko</name><affiliation wicri:level="1"><nlm:affiliation>a Inserm, U-1003, Equipe labellisée par la Ligue Nationale contre le cancer, Villeneuve d'Ascq, F-59655 France; Université des Sciences et Technologies de Lille (USTL) , Villeneuve d'Ascq , F-59655 France .</nlm:affiliation><country wicri:rule="zip">France</country><wicri:regionArea>F-59655 France</wicri:regionArea></affiliation></author></analytic><series><title level="j">Channels (Austin, Tex.)</title><idno type="e-ISSN">1933-6969</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recently, we reported the cloning and characterization of short isoform of the icilin-activated cold receptor TRPM8 channel in keratinocytes, dubbed eTRPM8. We demonstrated that eTRPM8 via fine tuning of the endoplasmic reticulum (ER) - mitochondria Ca(2+) shuttling regulates mitochondrial ATP and superoxide (O2(•-)) production and, thereby, mediates control of epidermal homeostasis by mild cold. Here, we provide additional information explaining why eTRPM8 suppression and TRPM8 stimulation both inhibit keratinocyte growth. We also demonstrate that stimulation of eTRPM8 with icilin may give rise to sustained oscillatory responses. Furthermore, we show that ATP-induced cytosolic and mitochondrial Ca(2+) responses are attenuated by eTRPM8 suppression. This suggests positive interplay between eTRPM8 and purinergic signaling pathways, what may serve to facilitate the ER-mitochondria Ca(2+) shuttling. Finally, we demonstrate that cold (25°C) induces eTRPM8-dependent superoxide-mediated necrosis of keratinocytes. Altogether, these results are in line with our model of eTRPM8-mediated cold-dependent balance between keratinocyte proliferation and differentiation.</div></front></TEI><affiliations><list><country><li>France</li></country></list><tree><country name="France"><noRegion><name sortKey="Bidaux, Gabriel" sort="Bidaux, Gabriel" uniqKey="Bidaux G" first="Gabriel" last="Bidaux">Gabriel Bidaux</name></noRegion><name sortKey="Borowiec, Anne Sophie" sort="Borowiec, Anne Sophie" uniqKey="Borowiec A" first="Anne-Sophie" last="Borowiec">Anne-Sophie Borowiec</name><name sortKey="Gordienko, Dmitri" sort="Gordienko, Dmitri" uniqKey="Gordienko D" first="Dmitri" last="Gordienko">Dmitri Gordienko</name><name sortKey="Prevarskaya, Natalia" sort="Prevarskaya, Natalia" uniqKey="Prevarskaya N" first="Natalia" last="Prevarskaya">Natalia Prevarskaya</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000038 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000038 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:27014839
|texte= Fine-tuning of eTRPM8 expression and activity conditions keratinocyte fate.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:27014839" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a OperaV1
| This area was generated with Dilib version V0.6.21. |  |